|
|
Gram
Positive
|
Gram
Negative
|
|
Peptidoglycan
content
|
>50%
|
10-20%
|
|
Cell wall
thickness
|
20-80 nm
|
10 nm
|
|
Layers of cell
wall
|
Many (40)
|
Few (1-2)
|
|
Teichoic acid
in cell wall
|
Present
|
Absent
|
|
Outer membrane
|
Absent
|
Present
|
|
Lipids and
lipoprotein
|
Few (0-3%)
|
Many (58%)
|
|
Lipopolysaccharides
|
Absent
|
Present
|
|
Digestion by
trypsin or pepsin
|
Resistant
|
Susceptible
|
|
Cell wall
digestion by lysozyme
|
Many species
|
Require
pre-treatment of cell wall
|
|
Dissolved by
1% NaOH
|
Resistant
|
Susceptible
|
|
Susceptibility
to anionic detergents
|
Marked
|
Much less
|
|
Susceptibility
to low surface tension
|
Marked
|
Much less
|
|
Inhibition by
Crystal violet
|
Marked
|
Much less
|
|
Susceptibility
to Penicillin
|
Marked
|
Much less
|
|
Periplasmic
space
|
Absent
|
Present
|
|
Gram reaction
|
Violet or
purple
|
Pink
|
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